Journal: PLoS ONE

Article Title: Three Dimensional Collagen Scaffold Promotes Intrinsic Vascularisation for Tissue Engineering Applications

doi: 10.1371/journal.pone.0149799

Figure Lengend Snippet: (A) A 3D collagen scaffold measured to a diameter of 8 mm and a thickness of 2 mm. (B) Representative images captured by scanning electron microscopy on the surface (left) and through a cross section (right) of collagen scaffold.

Article Snippet: Quantification of tissue vascularization and scaffold degradation were performed using video microscopy with a computer-assisted stereo investigator system (MBF Bioscience, VT, USA)[ , ].

Techniques: Electron Microscopy

Journal: PLoS ONE

Article Title: Three Dimensional Collagen Scaffold Promotes Intrinsic Vascularisation for Tissue Engineering Applications

doi: 10.1371/journal.pone.0149799

Figure Lengend Snippet: (A) Representative images captured from 3D collagen scaffolds seeded with human ASCs for 24 hours: haematoxylin and eosin (H&E) staining and scanning electron microscopy (SEM). The white arrow demonstrates human ASCs attached to the collagen scaffold. (B) Representative images of GFP-expressed human ASCs cultured in collagen scaffolds for 7 and 21 days. Scale bar = 200 μm. (C) The proliferation rate of GFP-expressed human ASCs cultured in 3D collagen scaffolds and on 2D collagen-coated plates (n = 3). ** p <0.01 vs 2D culture by unpaired t-test.

Article Snippet: Quantification of tissue vascularization and scaffold degradation were performed using video microscopy with a computer-assisted stereo investigator system (MBF Bioscience, VT, USA)[ , ].

Techniques: Staining, Electron Microscopy, Cell Culture